Journal: Frontiers in microbiology

Article Title: Enhancing epitope of PEDV spike protein.

doi: 10.3389/fmicb.2022.933249

Figure Lengend Snippet: FIGURE 1 Enhancement of the PEDV infectivity mediated by the mAbG3. (A) The mAbG3 at the concentrations of 5, 10, 20, and 40 μg was mixed with 100 pfu of PEDV GII strain P70 and incubated for 1 h before adding to the confluent monolayers of Vero cells. Immune serum to PEDV S1 subunit (1:100) and mAbA3 (40 μg) served as positive neutralization controls, medium alone served as negative neutralization/enhancement control, and isotype- matched IgG1-kappa mAb served as irrelevant mAb control. After 1 h of incubation, the fluid in each well was removed; the cells were rinsed and covered with 2% CMC in DMEM supplemented with 2 μg/ml of TPCK-treated trypsin. Two days post-infection, cells were fixed, and the number of syncytial formations was counted and calculated to % enhancement of PEDV infectivity compared to the medium alone. Data of each bar graph are presented as the mean and standard deviation of the % mAbG3-mediated enhancement of PEDV infectivity from triplicate wells of each treatment. (B) Results of qRT- PCR for determination of PEDV RNA recovered from different treatment groups. Y-axis, fold changes of PEDV RNA in different treatment groups compared to medium alone; X-axis, various treatment groups. By one-way ANOVA: ns, not significantly different; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. (C) Examples of the PEDV-mediated Vero cell CPE of different treatments. (D) Appearance of syncytial formation of PEDV-infected Vero cells (upper panel) compared with normal Vero cells (lower panel) at ×100 magnification of a light microscope.

Article Snippet: African green monkey kidney epithelial (Vero) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, United States).

Techniques: Infection, Incubation, Neutralization, Control, Standard Deviation, Quantitative RT-PCR, Light Microscopy

Journal:

Article Title: Extended Analysis of the In Vitro Tropism of Porcine Endogenous Retrovirus

doi:

Figure Lengend Snippet: Relative susceptibility of nonhuman cell lines to infection by PERV-NIH-2°-derived PERV pseudotypes

Article Snippet: 293 human embryonic kidney (ATCC CRL-1573), MMK Mus musculus molossinus kidney (ATCC CRL-6439; no longer available), and SC-1 mouse embryo epithelial (ATCC CRL-6450) cells, MDTF Mus dunni tail fibroblasts (kindly provided by Olivier Danos), NRK normal rat kidney cells (ATCC CRL-6509), Rat-2 rat embryo fibroblasts (ATCC CRL-1764), SIRC rabbit corneal fibroblasts (ATCC CCL-60), D17 canine oseosarcoma (ATCC CRL-6248), MDBK bovine kidney epithelium (ATCC CCL-22), FRhK4 rhesus monkey kidney (ATCC CRL-1688), Mv1Lu mink lung epithelial (ATCC CCL-64), and MiCL.1 (S+L−) mink lung (ATCC CCL-64.1) cells, Fc3TG feline tongue fibroblasts (ATCC CCL 176), and AK-D feline lung epithelial (ATCC CCL-150), CRFK feline kidney epithelial (ATCC CCL-94), CaKi-1 clear cell kidney carcinoma (ATCC HTB-46), HeLa cervical adenocarcinoma (ATCC CCL-2), HOS human osteosarcoma (ATCC CRL-1543), and CaCO-2 colorectal adenocarcinoma (ATCC HTB-37) cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 1 mM sodium pyruvate, 100 U of penicillin per ml, and 100 μg of streptomycin per ml.

Techniques: Infection, Control